Pseudombrophila ramosa

Pseudombrophila ramosa (Velen.) Brumm., Libri Botanici 14: 80. 1995. [pdf]

MycoBank MB413976

AUTHOR: Martinez-Gil R.

 

≡  Lachnea ramosa Velen., Monogr. Discom. Bohem. (Prague): 309. 1934. [basionym][pdf]

≡  Anthracobia ramosa (Velen.) Svrcek, Acta Mus. Nat. Prag. 4B (6): 74. 1948. [pdf]

− Ramulina ramosa (Velen.) Svrcek, Acta Mus. Nat. Prag. 4B (6): 74. 1948. [nom. inval. Art. 36.1, Ej. 7][pdf]

− Ramulina ramosa (Velen.) Velen., Novit. Mycol. Noviss.: 146-147 (1947) [nom. inval, Art. 35.2, the epithet “ramosa” was not positively associated with the genus Ramulina Vel.][pdf]

Taxonomic Classification

phy Ascomycota

sbp Pezyzomycotina

cls Pezizomycetes

sbc Pezizomycetidae

ord Pezizales

fam Pseudombrophilaceae

gen Pseudombrophila

sp Pseudombrophila ramosa

Description

Note: The study of all macro and microscopic morphological characters has been conducted from living apothecia.

Apothecia up to 5 mm in diameter, sessile, gregarious or scattered, initially cup-shaped, later discoid, flattened, and finally concave, with a somewhat depressed center and narrowing toward the base. Hymenium almost smooth, initially beige, later light brown, and finally reddish-brown, with a slightly dentate margin and slightly darker. The outer surface is furfuraceous, lighter than the rest, with numerous hyaline hyphae, especially on its lower part, which anchor them to the substrate.

Ascospores 11.5-13.3 × 7.5-8.3 µm, Q = 1.4-1.7, n = 47, ellipsoidal, hyaline, with some medium-sized guttules inside, tending to be located towards the poles, mononucleate, ornamented with a very irregular and difficult-to-observe fine network under the light microscope. Asci 160-200 × 13.5-18 μm, cylindrical, narrowing towards the base with a rounded apex, operculate, aporrincas, non-amyloid, with 8 uniseriate spores. Paraphyses 1.5-3 μm wide, slightly widening towards the apex, sometimes capitate, up to 5 μm thick, filiform, branched, septate, somewhat constricted at the septa, with a fair amount of reddish-brown external content. The medullary exciple has an intricate texture, formed by cylindrical, branched, disordered cells, 5-11 µm thick, interspersed with some globular or pear-shaped cells up to 20 µm in diameter, with clear beige vacuolar content. The ectal exciple has a globular texture, consisting of polyhedral, globular cells, light brown, 10-22 µm in diameter. Hairs are generated from cells of the ectal exciple, septate, formed by 3-10 cylindrical or broadly cylindrical elements, with abundant dark brown parietal content, 5-8 µm wide, with a club-shaped or fusiform apical section and a blunt apex. Hyphal hairs are more abundant at the base of the apothecia, cylindrical, hyaline, septate, interwoven, 2-4 µm wide, with a blunt apex and a thick refractive wall of 0.5-1.2 µm.

Ecology and Distribution: Originally described on rotten stems of Trifolium pratense L. According to the documents consulted, collections have been made from October to April on degraded plant remains without the presence of animal excrement or urea. The collection presented here was found on leaves and twigs of Quercus ilex subsp. ballota (Desf.) Samp., and also on remains of yellowish bryophytes partially hidden among the leaf litter.

Species References in the Area

We have only found data for one (unpublished) collection from Málaga, in the Navares/Tejares area (Ronda), 36.736444, -5.142417, 698 m, on the ground in a composting area with herbaceous remains and ashes, 13-XII-2020, leg. F.J. Valencia, CVL131220(1). [Data]

Specimens in Scientific Collections

Fig 1 Collections in Logroño and Málaga

* sequenced material

RM: private herbarium of R. Martínez-Gil; CVL: private herbarium of F.J. Valencia.

Reference specimen marked in bold

Spain

Lo: RM2787*.

Ma: CVL131220(1).

Sequences in Public Databases and Obtained from Territory Material

RM-2787: OR761983 (ITS), OR761984 (LSU)

 

Fig. 2. Pseudombrophila ramosa (RM-2787). Apothecia in situ. Photograph: R. Martínez-Gil.

Fig. 3. Pseudombrophila ramosa (RM-2787). Apothecia in situ. Photograph: R. Martínez-Gil.

Fig. 4. Pseudombrophila ramosa (RM-2787). Apothecia in situ. Photograph: R. Martínez-Gil.

Fig. 5. Pseudombrophila ramosa (RM-2787). A-B: Asci. C-D: Paraphyses. Microphotographs in water. Scale bars = 20 µm.

Fig. 6. Pseudombrophila ramosa (RM-2787). A-D: Spores from free spore print. A: Spores in water. B: Spore ornamentation in water. C-D: Spore ornamentation in blue cotton. Scale bars = 20 µm.

Fig. 7. Pseudombrophila ramosa (RM-2787). A: Medullary exciple. B: Ectal exciple. C: Subhymenium. D: Hyphal hairs. Microphotographs in water. Scale bars = 20 µm.

Fig. 8 Pseudombrophila ramosa (RM-2787). A-B: Apothecial section. C-F: Ectal exciple hairs. Microphotographs in water. Scale bars: A = 100 µm; B-F = 20 µm.

Acknowledgments

We would like to thank Uwe Lindemann and Francisco Javier Valencia López for their assistance in determining this species.

 

How to cite this sheet:

Martinez-Gil. R. 2023. Pseudombrophila ramosa (Velen.) Brumm. Fichas SIM 45  (updated on 3-XI-2023). DOI: https://doi.org/10.51436/SIMsheets/2023/45
Available at: https://micologiaiberica.org/en/services/Pseudombrophila ramosa